홈 > Research Lab > Lab of Pharmaceutical Biochemistry
홈 > Research Lab > Lab of Pharmaceutical Biochemistry
Education & Career
1977-1981: BS, College of Pharmacy, Chungbuk National University, Korea
1981-1983: MS, College of Pharmacy, Seoul National University, Korea
1984-1988: Ph.D, Dept. of Biochemical & Biophysical Sciences, University of Houston, USA
1988-1989: Post-Doc, Division of Applied Toxicology, MIT, USA
1989-1998: Assistant & Associate Professor, College of Pharmacy, Chungbuk National University
1998-present: Full professor, College of Pharmacy, Chungbuk National University
2004-2005: Dean, College of Pharmacy, Chungbuk National University
2006-2013: BK21 Director, Frontier Pharmaceutical Technology for Biotopia
2007-2008: Chairman, Division of Pharmaceutical Biochemistry, The Pharmaceutical Society of Korea
2008-2010: Director of Research Institute, Development of Pharmaceutical Resources, Chungbuk National University
2013-present: BK21+ Director, Frontier Pharmacy Leaders
2015-present: Vice President, The Pharmaceutical Society of Korea
International Collaboration & Short Visiting Abroad
2001. 7: Univ. of Tokyo, Japan (Prof. Kyioshi Takatsu & IL-5)
1998. 7: Tohoku Univ., Japan (Prof. Kazuo Ohuchi & Inflammatory signaling)
1997. 1: Chiba Univ., Japan (Prof. Shingo Yano & Hypersensitivity models)
1996. 8: Toyama Medical & Pharmaceutical Univ., Japan (Prof. Hideo Nakagawa & Chemokine)
1996. 1: Osaka Univ. School of Medicine, Japan (Prof. Toshio Hirano & IL-6)
1994. 7: Michigan State Univ., USA (Prof. Bill Smith & Prostanoids)
1) Drug Discovery Targeting Toll-like Receptor and Signal Transduction
2) Drug Discovery against Skin Hyperpigmentation
1) Park SH, Baek S-I, Yun J, Lee S, Yoon DY, Jung J-K, Jung S-H, Hwang BY, Hong JT, Han S-B, Kim Y. IRAK4 as a molecular target in the amelioration of innate immunity-related endotoxic shock and acute liver injury by chlorogenic acid. J. Immunol. 194: 1122-1130 (2015).
2) Shin H, Hong SD, Roh E, Jung S-H, Cho W-J, Park SH, Yoon DY, Ko SM, Hwang BY, Hong JT, Heo T-Y, Han S-B, Kim Y. cAMP-dependent PKA activation as a therapeutic target of skin hyperpigmentation by diphenylmethylene hydrazinecarbothioamide. Br. J. Pharmacol. 172: 3434-3445 (2015).
3) Roh E, Jeong I-Y, Shin H, Song S, Hong JT, Lee SH, Han S-B, Kim Y. Downregulation of melanocyte-specific facultative melanogenesis by 4-hydroxy-3-methoxycinnamaldehyde acting as a cAMP antagonist. J. Invest. Dermatol. 134: 551-553 (2014).
1) Kim Y, Hwang BY, Han S-B. Skin whitening composition comprising kobophenol A as active ingredient. Patent # 10-1481884 (2015-Jan-6).
2) Kim Y, Hwang BY. Novel daphene diterpenoid compound and composition for skin whitening comprising the same as active ingredient. Patent # 10-1467605 (2014-Nov-25).
3) Jung SH, Kim Y. Composition for whitening of the skin comprising thiosemicarbazone derivatives. Patent # 10-1248025 (2013-March-21).
1988-1992: BS, College of Pharmacy, Chungbuk National University
1992-1994: MS, College of Pharmacy, Chungbuk National University
1998-2001: Ph.D, Department of Biological Sciences, KAIST
1992-2007: Senior Research Scientist, KRIBB, Korea
2003-2004: Post Doc, NIAID, NIH, USA
2007-present: Professor, College of Pharmacy, Chungbuk National University
2011-2013: Chief, Department of Pharmacy, College of Pharmacy, Chungbuk National University
2015-2017: Vice dean, College of Pharmacy, Chungbuk National University
1) Rgs1 and Gnai2 regulate the entrance of B lymphocytes into lymph nodes and B cell motility within lymph node follicles. Han SB, Moratz C, Huang NN, Kelsall B, Cho H, Shi CS, Schwartz O, Kehrl JH. Immunity. 2005 Mar;22(3):343-54.
2) Tussilagone inhibits dendritic cell functions via induction of heme oxygenase-1. Park Y, Ryu HS, Lee HK, Kim JS, Yun J, Kang JS, Hwang BY, Hong JT, Kim Y, Han SB. Int Immunopharmacol. 2014 Oct;22(2):400-8.
3) Platycodon grandiflorum polysaccharide induces dendritic cell maturation via TLR4 signaling. Park MJ, Ryu HS, Kim JS, Lee HK, Kang JS, Yun J, Kim SY, Lee MK, Hong JT, Kim Y, Han SB. Food Chem Toxicol. 2014 Oct;72:212-20.
4) Saucerneol D inhibits dendritic cell activation by inducing heme oxygenase-1, but not by directly inhibiting toll-like receptor 4 signaling. Ryu HS, Lee HK, Kim JS, Kim YG, Pyo M, Yun J, Hwang BY, Hong JT, Kim Y, Han SB. J Ethnopharmacol. 2015 May 26;166:92-101.
1) Immune cell therapy of cancer
Natural killer (NK) cells are large granular lymphocytes capable of clearing both virus-infected and transformed cells. NK cell cytotoxicity is controlled by the integration of activating and inhibitory receptor signaling at the NK cell immune synapse (IS) formed between NK and target cells. NK cells can also respond by producing cytokines, e.g., interferon-γ (IFN-γ) or tumor necrosis factor-α (TNF-α), and are known to be activated by cytokines like interleukin (IL)-2, IL-12, and IL-15. Activating receptors, such as DNAM-1, NKp44, and KLRB1, are upregulated, while inhibitory receptors, like KIR2DL2 and KIR3DL3, are downregulated after exposure to IL-2. In addition, increased cell-cell adhesion has been directly coupled to cytotoxicity. NK cells are able to lyse target cells but require the right combination of activating signals, and, therefore, seem more tightly regulated than IL-2-activated NK cells. In our lab, we are focusing on the identification of cytotoxic dynamics of NK cells. Using time-lapse imaging, we examine "cytotoxic dynamics", such as killing behavior, contact dynamics, motility and directionality of NK cells as well as dying process of cancer cells.
2) Mesenchymal stem cell therapy of autoimmune diseases
Mesenchymal stem cells (MSCs) are present in diverse tissues and organs, including bone marrow, umbilical cord, adipose tissue, and placenta. MSCs can expand easily in vitro and have regenerative stem cell properties and potent immunoregulatory activity. They inhibit the functions of dendritic cells, B cells, and T cells, but enhance those of regulatory T cells by producing immunoregulatory molecules such as transforming growth factor-β, hepatic growth factors, prostaglandin E2, interleukin-10, indolamine 2,3-dioxygenase, nitric oxide, heme oxygenase-1, and human leukocyte antigen-G. These properties make MSCs promising therapeutic candidates for the treatment of autoimmune diseases. However, the detailed mechanisms by which MSCs exert their immunomodulatory functions are still incompletely understood. In our lab, we focus on identifying the immunoregulatory mechanisms of MSCs. Our hypothesis is that MSCs might regulate the functions of immune cells (T cells, B cells, dendritic cells, and so on) via producing soluble mediators and direct cell-cell contact. To prove it, two strategies are established. First, we are trying to identify cell-type-specific soluble mediator. Second, using time-lapse imaging, we are studying the cell type-specific contact dynamics, such as contact duration, contact frequency, motility speeds, directionality, and their relevance with chemokine axis.
3) ) Immuno- & Onco-pharmacology
Our lab has assay systems to evaluate the efficacy of immunomodulators (stimulants and suppressants). In enzymatic assay, we general identify the direct molecular target of compounds by using kinase assay. In cellular assay, we generally test the effect of compounds on viability, proliferation and Ab production of B cells, proliferation and cytokine production of T cells, maturation of dendritic cells (6 assay parameters), and cytokine production of macrophages. In addition, we examine the effect of compounds on signaling pathways downstream from TCR, BCR, TLRs, and cytokine receptors. In animal assay, we have syngeneic tumor model (tumor growth and metastasis) to develop immunostimulants and inflammation models (lupus and rheumatoid arthritis) to develop anti-inflammatory drugs. We also have assay systems to evaluate the efficacy and mechanisms of anti-cancer drug candidates. In enzymatic assay, we general identify the direct molecular target of compounds by using kinase assay. In cellular assays, we examine the cytotoxic potential of compounds on more than 60 human cancer cell lines by using SRB, MTT, and SRB assay (for tumor growth inhibitor). We also examine the invasion and migration of endothelial cells (for angiogenesis blocker) and cancer cells (for metastasis inhibitor). We used qRT-PCR to examine the effects of compounds on gene expression and WB on protein expression/phosphorylation. In animal assay, we have syngeneic murine tumor model, xenograft human tumor model (nude mice), and lung metastasis models.